منابع مشابه
Assessment of the microbial diversity of Brazilian kefir grains by PCR-DGGE and pyrosequencing analysis.
The microbial diversity and community structure of three different kefir grains from different parts of Brazil were examined via the combination of two culture-independent methods: PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and pyrosequencing. PCR-DGGE showed Lactobacillus kefiranofaciens and Lactobacillus kefiri to be the major bacterial populations in all three grains. The yeast c...
متن کاملPCR-DGGE fingerprinting: novel strategies for detection of microbes in food.
Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. This paper describes the technique and reports on the state-of-the-art application of this technique to food and food-related ecosystems. Applications of PCR-DGGE in several fields of food microbiology are reviewed: the identification of microorganisms isol...
متن کاملAnalysis of microbial diversity in Shenqu with different fermentation times by PCR-DGGE
Shenqu is a fermented product that is widely used in traditional Chinese medicine (TCM) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. The aim of this study was to investigate microbial diversity in Shenqu using different fermentation time periods. DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated tha...
متن کاملApproach to determine the diversity of Legionella species by nested PCR-DGGE in aquatic environments
In this study, we describe a nested PCR-DGGE strategy to detect Legionella communities from river water samples. The nearly full-length 16S rRNA gene was amplified using bacterial primer in the first step. After, the amplicons were employed as DNA templates in the second PCR using Legionella specific primer. The third round of gene amplification was conducted to gain PCR fragments apposite for ...
متن کاملFungal diversity in grape must and wine fermentation assessed by massive sequencing, quantitative PCR and DGGE
The diversity of fungi in grape must and during wine fermentation was investigated in this study by culture-dependent and culture-independent techniques. Carignan and Grenache grapes were harvested from three vineyards in the Priorat region (Spain) in 2012, and nine samples were selected from the grape must after crushing and during wine fermentation. From culture-dependent techniques, 362 isol...
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ژورنال
عنوان ژورنال: Journal of the Korean Fisheries Society
سال: 2007
ISSN: 0374-8111
DOI: 10.5657/kfas.2007.40.6.356